李正强
教授 博士生导师
lzq@jlu.edu.cn
电话:0431-85155201
传真:+86-431-85155200
分子酶学工程教育部重点实验室主任
吉林大学生命科学学院副院长
 
 
人员介绍
学历:
1984年06月 吉林大学理学学士(生物化学)
1990年06月 吉林大学理学硕士(生物化学)
1994年06月 吉林大学理学博士(物理化学)

任职经历:
1995-1996年 香港科技大学化学系 博士后
1997-1999年 吉林大学超分子结构与材料国家重点实验室副教授
2000-2001年 日本岗崎国立共同研究机构分子科学研究所
    文教科学省聘任外国人研究员
2001年9月 吉林大学分子酶学工程教育部重点实验室 教授 学术兼职;

学术兼职:
中国生物物理学会理事
中国微生物学会酶工程专业委员会委员
吉林省生物质资源利用研究会副理事长
吉林省生物化学与分子生物学学会常务理事
吉林省食品学会常务理事
吉林省药学会保健食品专业委员会常务委员

研究方向
研究领域分支为酶学、结构生物学、分子生物物理学、无机-生物化学等
研究方向:酶学与酶组装技术
主要涉及:
(1)血红素辅基酶(蛋白质)的结构表征及功能分子调控、结构模拟
(2)活性气体小分子与心脑血管功能调控的酶学基础;
(3)酶与无机复合材料组装、功能及应用研究。

承担项目
1. 烷基化一氧化氮调控可溶性鸟苷酸环化酶的结构基础与分子机制研究 国家自然科学基金面上项目(31670797)2017.01-2020.12
2. 印染用酶的分子改造研究 国家高技术研究发展计划“863”计划项目(2012AA022202B)2012.11-2015.12
3. 基于表面增强共振拉曼光谱技术的脱氧核酶构效关系研究 国家自然科学基金面上项目(30870533)2009.01--2011.12
4. 基于“Fish-in-Net”方法的双酶共固定技术研究 教育部博士点基金(20100061110028)2011.1-2013.12
5. 蒜源SAC可控转化为硫化氢抗心肌缺血的临床前研究 吉林省科技发展计划项目科技支撑计划(2 0 1 2 0 9 4 6)2012.1-2014.12
6. 硅基介孔材料包封菌技术提高生物乙醇转化过程碳有效利用度的研究 吉林省科技厅社会发展研究项目(20090404) 2009.08-2011.12

文章、专利、著作等
1. Proximal environment controlling the reactivity between inorganic sulfide and heme-peptide model. RSC Adv., (2016)6: 78858-78864.
2. Entry of water into the distal heme pocket of soluble Guanylate Cyclase β1 H-NOX domain alters the ligated CO structure: A resonance Raman and in silico simulation study. RSC Adv., (2016)6: 43707-43714.
3. Nonresonant chemical mechanism in surface-enhanced Raman scattering of pyridine on M@Au12 clusters. Nanoscale, (2016) 8: 4086-4093.
4. Amino acids-incorporated nanoflowers with an intrinsic peroxidase-like activity. Scientific Reports. (2016)6: 22412. 5. Clues for discovering a new biological function of Vitreoscilla hemoglobin in organisms: a potential sulfide receptor and storage. FEBS Letters, (2016)590: 1132–1142.
6. Immobilization of Lactobacillus rhamnosus in mesoporous silica-based material:An efficiency continuous cell-recycle fermentation system for lactic acid production. Journal of Bioscience and Bioengineering, (2016)6: 645-651.
7. Raman spectroscopic characteristics of diallyl trisulfide acting ontrans-crotonaldehyde. J. Raman Spectroscopy, (2015)46: 1067-1072.
8. Construction and Characterization of Vitreoscilla Hemoglobin (VHb) with Enhanced Peroxidase Activity for Efficient Degradation of Textile Dye. J. Microbiol. Biotechnol., (2015)25(9): 1433-1441.
9. The process of S-nitrosation in sGC β1(1–194) revealed by infrared spectroscopy. Journal of Molecular Structure, (2015)1089: 102-106.
10. Strong Core@Shell Dependence in Surface-Enhanced Raman Scattering of Pyridine on Stable 13-Atom Silver-Caged Bimetallic Clusters. J. Phys. Chem. C, (2015)119: 17429-17437.
11. UV-visible and Raman Spectroscopic Studies of Lithocholic Acid on E-2-Butenal for Anti-Glioma. Spectroscopy Letters, (2015)48: 506-513.
12. The mechanism of N-Ag bonding determined tunability of surface-enhanced Raman scattering of pyridine on MAg (M = Cu, Ag, Au) diatomic clusters. Phys. Chem. Chem. Phys., (2014)16: 20665-20671.
13. Interactions of caveolin-1 scaffolding and intramembrane regions containing a CRAC motif with cholesterol in lipid bilayers. Biochimica et Biophysica Acta, (2014)1838: 2588-2599.
14. Resolution of 1,1,1-trifluoro-2-octanol by Pseudomonas sp. lipase encapsulated in aggregated silica nanoparticles. RSC Adv., (2014)4: 6103-09.
15. Spectroscopic Studies on the Interaction Between Aucubin and Bovine Serum Albumin without or with Copper II or Iron III. Spectroscopy Letters, (2014)47: 119-130.
16. “Fish-in-Net“, a Novel Method for Cell Immobilization of Zymomonas mobilis. PLOS ONE, (2013)11:e79569.
17. pH-induced quaternary assembly of Vitreoscilla hemoglobin: The monomer exhibits better peroxidase activity. BBA-Proteins and Proteomics, (2013)1834: 2124-32.
18. Theoretical investigation on the diatomic ligand migration process and ligand binding properties in non-O2-binding H-NOX domain. Proteins: Structure, Function and Bioinformatics, (2013)81:1363-76.
19. Characterization of the Interaction between Eupatorin and Bovine Serum Albumin by Spectroscopic and Molecular Modeling Methods. Int J Mol Sci., (2013)14:14185-203.
20. How does (E)-2-(acetamidomethylene) succinate bind to its hydrolase? From the binding process to the final result., PLoS One, (2013)8:e53811.
21. Chemical Mechanism and Tunability of Surface-enhanced Raman Scattering of Pyridine on Heteronuclear Coinage Metal Diatomic Clusters: A Density Functional Study. J. Phys. Chem. C, (2013)117: 12544-12551.
22. Improving the properties of β-galactosidase from Aspergillus oryzae via encapsulation in aggregated silica nanoparticles. New.J.Chem., (2013)37: 3793-3797.
23. Improvement of the Enzyme Performance of Trypsin via Adsorption in Mesoporous Silica SBA-15: Hydrolysis of BAPNA, Molecules, (2013)18: 1138-1149.
24. Molecular Dynamics Simulations Suggest Ligand’s Binding to Nicotinamidase/Pyrazinamidase. PLoS ONE, (2012)7:e39546
25. Hemoglobin immobilized with modified “fish-in-net” approach for the catalytic removal of aniline. Journal of Hazardous Materials, (2012)217-218: 156-163.
26. Combining the Physical Adsorption Approach and the Covalent Attachment Method to Prepare a Bifunctional Bioreactor. Int. J. Mol. Sci., (2012)13: 11443-11454.

专著:
常用酶活力测定方法 吉林大学出版社 2014年9月
酶工程 (第三版) 化学工业出版社 2016年5月

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